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KMID : 0380020200350010064
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Korean Journal of Biotechnology and Bioengineering
2020 Volume.35 No. 1 p.64 ~ p.71
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Antibacterial Activities of Strain Pantoea sp. S3W-11 and Optimization of Culture Conditions
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Nam Young-Ho
Choi Ah-Young
Chung Eu-Jin
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Abstract
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In this study, 46 bacteria strains were isolated from water and soil collected from Geumjin-ri, Ganggu-ri and Namseock-ri in Yeongdeok-Gun to screen antibacterial bacteria against various pathogenic bacteria. Among them, strain S3W-11 showed an excellent growth inhibition against methicillin-resistant Staphylococcus aureus, Bacillus cereus and Escherichia coli. As a result of the 16S rRNA gene sequence analysis, strain S3W-11 showed the high similarity with Pantoea vagans LMG 24199T, Pantoea agglomerans DSM 3493T, Pantoea conspicua LMG 24534T 99.64%, 99.58%, 99.18%, respectively. The effects of culture medium composition, temperature, and initial pH on the cell growth and antibacterial activity were confirmed for culture optimization of strain S3W-11. Optimal conditions for growth and antibacterial activity in strain S3W-11 were found to be: YPD medium, 25oC and pH 6.5. When the strain was cultured in LB, NB, TSB, and R2A media, the antibacterial activity has not been confirmed. Optimal reaction time was 48 h in YPD medium, 100 rpm and 0.3 vvm in 2 L-scale fed-batch fermentation process for antimicrobial activity. Strain S3W-11 can be improved on antibacterial activity in optimal culture conditions (YPD medium, 25oC and pH 6.5). Therefore, the antibacterial activity of Pantoea sp. S3W-11 had potential as antibiotics for pathogens including MRSA.
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KEYWORD
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Antibacterial activity, Pantoea sp. S3W-11, methicillin-resistant Staphylococcus aureus (MRSA)
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